pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W | Addgene 32521 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W

catalog :

32521

product information

Catalog Number :

32521

Product Name :

pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W

article :

doi
id	4690
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pHAGE-CMV-Rheb-IRES-eGFP-W

backbone_mutation

backbone_origin

Addgene plasmid #32519

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The published dominant negative D60I mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W by site directed mutagenesis. Although reported to have robust DN activity in other cell types, this construct did not provide robust DN activity in neurons. The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NotI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Rheb
id	26954

genbank_ids

mutation

D60I to make dominant negative

name

Dominant negative Rheb (D60I)

shRNA_sequence

size

species

10116

Rattus norvegicus