pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W | Addgene 32520 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

catalog :

32520

citations: 3

Reference
Yang F, Sun S, Wang C, Haas M, Yeo S, Guan J. Targeted therapy for mTORC1-driven tumours through HDAC inhibition by exploiting innate vulnerability of mTORC1 hyper-activation. Br J Cancer. 2020;122:1791-1802 pubmed publisher
Simonson B, Subramanya V, Chan M, Zhang A, Franchino H, Ottaviano F, et al. DDiT4L promotes autophagy and inhibits pathological cardiac hypertrophy in response to stress. Sci Signal. 2017;10: pubmed publisher
Nie D, Di Nardo A, Han J, Baharanyi H, Kramvis I, Huynh T, et al. Tsc2-Rheb signaling regulates EphA-mediated axon guidance. Nat Neurosci. 2010;13:163-72 pubmed publisher

product information

Catalog Number :

32520

Product Name :

pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

article :

doi	10.1038/nn.2477
id	4689
pubmed_id	20062052

bacterial resistance :

Ampicillin

cloning :

backbone

pHAGE-CMV-Rheb-IRES-eGFP-W

backbone_mutation

backbone_origin

Addgene plasmid #32519

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The constitutively activating S16H mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W (Addgene plasmid #32519) by site directed mutagenesis. The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NotI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Rheb
id	26954

genbank_ids

mutation

S16H to make constitutively active

name

Constitutively active Rheb (S16H)

shRNA_sequence

size

species

10116

Rattus norvegicus