pHAGE-CMV-Rheb-IRES-eGFP-W | Addgene 32519 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHAGE-CMV-Rheb-IRES-eGFP-W

catalog :

32519

citations: 1

Reference
Nie D, Di Nardo A, Han J, Baharanyi H, Kramvis I, Huynh T, et al. Tsc2-Rheb signaling regulates EphA-mediated axon guidance. Nat Neurosci. 2010;13:163-72 pubmed publisher

product information

Catalog Number :

32519

Product Name :

pHAGE-CMV-Rheb-IRES-eGFP-W

article :

doi	10.1038/nn.2477
id	4689
pubmed_id	20062052

bacterial resistance :

Ampicillin

cloning :

backbone

pHAGE-CMV-eGFP-IRES-eGFP-W

backbone_mutation

backbone_origin

backbone_size

8450

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Cloning of Rheb into the NotI and BamHI sites of pHAGE-CMV-eGFP-IRES-eGFP-W replaces the first copy of eGFP. The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	NotI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Rheb
id	26954

genbank_ids

mutation

name

Rheb

shRNA_sequence

size

species

10116

Rattus norvegicus