This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D
catalog :
32457
citations: 1
Reference
Green M, Scott J, Steel R, Oakhill J, Kemp B, Means A. Ca2+/Calmodulin-dependent protein kinase kinase beta is regulated by multisite phosphorylation. J Biol Chem. 2011;286:28066-79 pubmed publisher
product information
Catalog Number :
32457
Product Name :
pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D
article :
doi10.1074/jbc.M111.251504
id4665
pubmed_id21669867
bacterial resistance :
Ampicillin
cloning :
backbonepSG5
backbone_mutationAdded blunt end cloning site which was lost during cloning.
backbone_originStratagene
backbone_size4100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The S3V, G96R, and P108L mutations were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
origin :
37
pi :
alt_names
CaMKKbeta
CaMKK2
cloning
clone_methodRestriction Enzyme
cloning_site_3None
cloning_site_5None
promoterSV40
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliases
geneCamkk2
id83506
genbank_ids
NM_031338
mutationS129D, S133D, S137D (S3V, G96R, and P108L also present)
nameCalcium/Calmodulin dependent protein kinase kinase beta
shRNA_sequence
size1700
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagFLAG
resistance markers :
1129
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA