This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-N1-CaMKKbeta S129D, S133D, S137D
catalog :
32455
citations: 1
Reference
Green M, Scott J, Steel R, Oakhill J, Kemp B, Means A. Ca2+/Calmodulin-dependent protein kinase kinase beta is regulated by multisite phosphorylation. J Biol Chem. 2011;286:28066-79 pubmed publisher
product information
Catalog Number :
32455
Product Name :
pEGFP-N1-CaMKKbeta S129D, S133D, S137D
article :
doi10.1074/jbc.M111.251504
id4665
pubmed_id21669867
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutationFLAG tag added into NheI site by cutting and inserting linker with FLAG tag and NheI overhangs. Second NheI site removed by site-directed mutagenesis.
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
W374C mutation present.
origin :
37
pi :
alt_names
CaMKKbeta
CaMKK2
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NheI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliases6330570N16Rik, AW061083, mKIAA0787
geneCamkk2
id207565
genbank_ids
NM_145358.1
mutationS129D, S133D, S137D
nameCalcium/Calmodulin dependent protein kinase kinase beta
shRNA_sequence
size
species
10090
Mus musculus
tags
locationC terminal on backbone
tagGFP
locationN terminal on insert
tagFLAG
resistance markers :
1129
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA