pSG5-FLAG-CaMKKbeta rat D311A | Addgene 32451 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSG5-FLAG-CaMKKbeta rat D311A

catalog :

32451

citations: 1

Reference
Anderson K, Ribar T, Lin F, Noeldner P, Green M, Muehlbauer M, et al. Hypothalamic CaMKK2 contributes to the regulation of energy balance. Cell Metab. 2008;7:377-88 pubmed publisher

product information

Catalog Number :

32451

Product Name :

pSG5-FLAG-CaMKKbeta rat D311A

article :

doi	10.1016/j.cmet.2008.02.011
id	4664
pubmed_id	18460329

bacterial resistance :

Ampicillin

cloning :

backbone

pSG5

backbone_mutation

Blunt end site added and destroyed during cloning. Nhe1 site added to 5' end of coding region. FLAG tag added into Nhe1 site which is conserved.

backbone_origin

Stratagene

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The S3V, G96R, and P108L mutations were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.

origin :

pi :

alt_names

CaMKKbeta

CaMKK2

cloning

clone_method	Restriction Enzyme
cloning_site_3	None
cloning_site_5	None
promoter	SV40
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	Camkk2
id	83506

genbank_ids

NM_031338

mutation

D311A (S3V, G96R, and P108L also present)

name

Calcium/Calmodulin dependent protein kinase kinase beta

shRNA_sequence

size

1700

species

10116

Rattus norvegicus