This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28a-At-TRL
catalog :
32242
citations: 2
Reference
Olzog V, Gärtner C, Stadler P, Fallmann J, Weinberg C. cyPhyRNA-seq: a genome-scale RNA-seq method to detect active self-cleaving ribozymes by capturing RNAs with 2',3' cyclic phosphates and 5' hydroxyl ends. RNA Biol. 2021;18:818-831 pubmed publisher
Schutz K, Hesselberth J, Fields S. Capture and sequence analysis of RNAs with terminal 2',3'-cyclic phosphates. RNA. 2010;16:621-31 pubmed publisher
product information
Catalog Number :
32242
Product Name :
pET28a-At-TRL
article :
doi10.1261/rna.1934910
id4615
pubmed_id20075163
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_originNovagen
backbone_size5255
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
We have found that this protein expresses best in the BL21 (DE3) RIPL cells from Stratagene. Standard BL21 (DE3) strains that lack tRNAs for rare codons do not work well (or at all).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NheI
promoterT7 RNA Polmyerase
sequencing_primer_3T7 Terminator
sequencing_primer_5T7 Promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G07910, ARABIDOPSIS THALIANA RNA LIGASE, ATRNL, AtRLG1, RNAligase, T6D22.1, T6D22_1, TRNA LIGASE
geneRNL
id837306
genbank_ids
mutation
nameA. thaliana tRNA ligase
shRNA_sequence
size3300
species
3702
Arabidopsis thaliana
tags
locationN terminal on backbone
tag6xHistidine
plasmid copy :
Hildburg Beier
resistance markers :
1441
1440
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA