LHP585 - Ub-CYC1 | Addgene 32168 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LHP585 - Ub-CYC1

catalog :

32168

citations: 1

Reference
Shih S, Prag G, Francis S, Sutanto M, Hurley J, Hicke L. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J. 2003;22:1273-81 pubmed

product information

Catalog Number :

32168

Product Name :

LHP585 - Ub-CYC1

article :

doi	10.1093/emboj/cdg140
id	4659
pubmed_id	12628920

bacterial resistance :

Ampicillin

cloning :

backbone

YEplac195

backbone_mutation

Contains CUP1 promoter, synthetic yeast ubiquitin, and CYC1 terminator sequence - all cloned in at same time as described in comments.

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

A ~1040 bp BamHI / HindIII fragment containing the CUP1 promoter, the synthetic yeast Ub gene and a CYC1 terminator sequence was ligated into the BamHI and HindIII sites of YEplac195. The resulting plasmid is approximately 6.281kb). 2 m S. cerevisiae replication.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	none
cloning_site_5	none
promoter	CUP1
sequencing_primer_3	LacZ-R
sequencing_primer_5	CUP1 forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YKL210W
gene	UBA1
id	853670

aliases	YDR390C, UAL1
gene	UBA2
id	851998

genbank_ids

mutation

name

uba1 yeast

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae