LHP809 - Ub-K63A-CYC1 | Addgene 32166 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LHP809 - Ub-K63A-CYC1

catalog :

32166

product information

Catalog Number :

32166

Product Name :

LHP809 - Ub-K63A-CYC1

article :

doi
id	4600
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

YEplac195

backbone_mutation

Contains CUP1 promoter, synthetic yeast ubiquitin, and CYC1 terminator sequence - all cloned in at same time as described in comments. K63A mutation was introduced by site-directed mutagenesis of LHP585 (Addgene 32168).

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

A ~1040 bp BamHI / HindIII fragment containing the CUP1 promoter, the synthetic yeast Ub gene and a CYC1 terminator sequence was ligated into the BamHI and HindIII sites of YEplac195. The resulting plasmid is approximately 6.281kb). K63A mutation was introduced by site-directed mutagenesis of LHP585 (Addgene 32168). 2 m S. cerevisiae replication.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	none
cloning_site_5	none
promoter	CUP1
sequencing_primer_3	LacZ-R
sequencing_primer_5	CUP1 forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YKL210W
gene	UBA1
id	853670

aliases	YDR390C, UAL1
gene	UBA2
id	851998

genbank_ids

mutation

K63A

name

synthetic yeast Ubiquitin

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae