LHP306 - Ub - no Lys | Addgene 32150 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

LHP306 - Ub - no Lys

catalog :

32150

citations: 1

Reference
Terrell J, Shih S, Dunn R, Hicke L. A function for monoubiquitination in the internalization of a G protein-coupled receptor. Mol Cell. 1998;1:193-202 pubmed

product information

Catalog Number :

32150

Product Name :

LHP306 - Ub - no Lys

article :

doi	10.1016/S1097-2765(00)80020-9
id	4597
pubmed_id	9659916

bacterial resistance :

Ampicillin

cloning :

backbone

pTER62

backbone_mutation

The c-myc epitope tag was removed

backbone_origin

Mike Ellison - University of Alberta

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

Ub-no Lys; CUP1-Ub K6R, K11R, K27R, K29R, K33A, K48R, K63R (all Lys Changed to Arg); BamHI-BglII UBI fragment from YEp96 (D. Ecker, U PENN) was cloned into pTER62 (Mike Ellison, University of Alberta) cut with BamHI and BglII. 2 m S. cerevisiae origin

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	BamHI
promoter	CUP1
sequencing_primer_3
sequencing_primer_5	CUP1 forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YKL210W
gene	UBA1
id	853670

aliases	YDR390C, UAL1
gene	UBA2
id	851998

genbank_ids

mutation

No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

name

synthetic yeast Ubiquitin

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae