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product summary
company name :
Addgene
product type :
cDNA
product name :
pMAL-c2x-MT3
catalog :
32115
citations: 1
product information
Catalog Number :
32115
Product Name :
pMAL-c2x-MT3
article :
| doi | 10.1016/j.jsb.2008.11.009 |
| id | 4587 |
| pubmed_id | 19114107 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pMAL-c2x | |
| backbone_mutation | Already has two copies of MT inserted to begin with. This parent plasmid is available as Addgene plasmid 32089. | |
| backbone_origin | New England Biolabs | |
| backbone_size | 6700 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
which codes for the MBP linker region containing an XmnI and NcoI site fused in frame to the MT gene, and the reverse primer (50-GTGACCACATGTCACAGCACGTGCACTTGTCC-3 0 ), which contains an AflIII site at the MT MT junction. The pMAL-c2x-MT2 plasmid was prepared by digesting with XmnI and NcoI, and the PCR product was constructed with XmnI and AflIII. Since NcoI and AflIII produced equivalent DNA ends, the second copy of MT was inserted with removal of both restriction sites at the ligation junction. This process can be reproduced iteratively to introduce as many copies of MT in frame into the construct as desired. In addition to this plasmid Addgene offers versions with 1 copy (32101), 2 copies (32102) and 4 copies (32100) of MT.
(50-CTCGGGATCGAGGGAAGGATTTCAAGATATACCAT
GGACCCC-30),
In this construct, a translational modification was made that changes an alanine to an aspartic acid at the junction between MT genes. The third copy of the MT gene was inserted into the pMAL-c2x-MT2 plasmid (Addgene plasmids 32102) before the existing MT copies. The inserted MT gene was amplified from pET-3d-MT using the forward primer
(50-CTCGGGATCGAGGGAAGGATTTCAAGATATACCAT
GGACCCC-30),
In this construct, a translational modification was made that changes an alanine to an aspartic acid at the junction between MT genes. The third copy of the MT gene was inserted into the pMAL-c2x-MT2 plasmid (Addgene plasmids 32102) before the existing MT copies. The inserted MT gene was amplified from pET-3d-MT using the forward primer
origin :
37
pi :
|
plasmid copy :
The metallothionein (MT) gene was amplified from pET-3d-MT (a gift from the Winge laboratory, University of Utah), which contains the MT gene within the pET-3d vector (Novagen, Madison, WI).
resistance markers :
| 1036 |
| 1039 |
| 1037 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments