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product summary
company name :
Addgene
product type :
cDNA
product name :
pMAL-c2x-MT2
catalog :
32102
citations: 2
| Reference |
|---|
Mercogliano C, Derosier D. Concatenated metallothionein as a clonable gold label for electron microscopy. J Struct Biol. 2007;160:70-82 pubmed
|
product information
Catalog Number :
32102
Product Name :
pMAL-c2x-MT2
article :
| doi | 10.1016/j.jsb.2007.06.010 |
| id | 4589 |
| pubmed_id | 17692533 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pMAL-c2x | |
| backbone_mutation | Already has one copy of MT inserted to begin with. This parent plasmid is available as Addgene plasmid 32101. | |
| backbone_origin | New England Biolabs | |
| backbone_size | 6700 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
which codes for the MBP linker region containing an XmnI and NcoI site fused in frame to the MT gene, and the reverse primer (50-GTGACCACATGTCACAGCACGTGCACTTGTCC-3 0 ), which contains an AflIII site at the MT MT junction. The pMAL-c2x-MT plasmid was prepared by digesting with XmnI and NcoI, and the PCR product was constructed with XmnI and AflIII. Since NcoI and AflIII produced equivalent DNA ends, the second copy of MT was inserted with removal of both restriction sites at the ligation junction. This process can be reproduced iteratively to introduce as many copies of MT in frame into the construct as desired. In addition to this plasmid Addgene offers versions with 1 copy (32101), 3 copies (32115) and 4 copies (32100) of MT.
(50-CTCGGGATCGAGGGAAGGATTTCAAGATATACCAT
GGACCCC-30),
In this construct, a translational modification was made that changes an alanine to an aspartic acid at the junction between the two MT genes. The second copy of the MT gene was inserted into the pMAL-c2x-MT1 plasmid (Addgene plasmids 32101) before the existing MT copy. The inserted MT gene was amplified from pET-3d-MT using the forward primer
(50-CTCGGGATCGAGGGAAGGATTTCAAGATATACCAT
GGACCCC-30),
In this construct, a translational modification was made that changes an alanine to an aspartic acid at the junction between the two MT genes. The second copy of the MT gene was inserted into the pMAL-c2x-MT1 plasmid (Addgene plasmids 32101) before the existing MT copy. The inserted MT gene was amplified from pET-3d-MT using the forward primer
origin :
37
pi :
|
plasmid copy :
The metallothionein (MT) gene was amplified from pET-3d-MT (a gift from the Winge laboratory, University of Utah), which contains the MT gene within the pET-3d vector (Novagen, Madison, WI).
resistance markers :
| 1036 |
| 1039 |
| 1037 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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