This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO-puro FLAG SREBP2
catalog :
32018
citations: 12
Reference
Firpo M, LoMascolo N, Petit M, Shah P, Mounce B. Polyamines and eIF5A hypusination facilitate SREBP2 synthesis and cholesterol production leading to enhanced enterovirus attachment and infection. PLoS Pathog. 2023;19:e1011317 pubmed publisher
Mahmud I, Tian G, Wang J, Hutchinson T, Kim B, Awasthee N, et al. DAXX drives de novo lipogenesis and contributes to tumorigenesis. Nat Commun. 2023;14:1927 pubmed publisher
Hunt B, Davis J, Fox L, Vicente Mu xf1 oz S, Lester C, Wells S, et al. RON-augmented cholesterol biosynthesis in breast cancer metastatic progression and recurrence. Oncogene. 2023;42:1716-1727 pubmed publisher
Tang W, Thundyil J, Lim G, Tng T, Yeow S, Nair A, et al. Parkin regulates neuronal lipid homeostasis through SREBP2-lipoprotein lipase pathway-implications for Parkinson's disease. Hum Mol Genet. 2023;32:1466-1482 pubmed publisher
Mai S, Zhu X, Wan E, Wu S, Yonathan J, Wang J, et al. Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2. Development. 2022;149: pubmed publisher
Shin S, Zhou H, He C, Wei Y, Wang Y, Shingu T, et al. Qki activates Srebp2-mediated cholesterol biosynthesis for maintenance of eye lens transparency. Nat Commun. 2021;12:3005 pubmed publisher
Gabitova Cornell L, Surumbayeva A, Peri S, Franco Barraza J, Restifo D, Weitz N, et al. Cholesterol Pathway Inhibition Induces TGF-β Signaling to Promote Basal Differentiation in Pancreatic Cancer. Cancer Cell. 2020;38:567-583.e11 pubmed publisher
Zhang J, Guo Y, Ge W, Zhou X, Pan M. High glucose induces the apoptosis of HUVECs in mitochondria dependent manner by enhancing VDAC1 expression. Pharmazie. 2018;73:725-728 pubmed publisher
Kim J, Garcia Carbonell R, Yamachika S, Zhao P, Dhar D, Loomba R, et al. ER Stress Drives Lipogenesis and Steatohepatitis via Caspase-2 Activation of S1P. Cell. 2018;175:133-145.e15 pubmed publisher
Zhang D, Tomisato W, Su L, Sun L, Choi J, Zhang Z, et al. Skin-specific regulation of SREBP processing and lipid biosynthesis by glycerol kinase 5. Proc Natl Acad Sci U S A. 2017;114:E5197-E5206 pubmed publisher
Ricoult S, Yecies J, Ben Sahra I, Manning B. Oncogenic PI3K and K-Ras stimulate de novo lipid synthesis through mTORC1 and SREBP. Oncogene. 2016;35:1250-60 pubmed publisher
Peterson T, Sengupta S, Harris T, Carmack A, Kang S, Balderas E, et al. mTOR complex 1 regulates lipin 1 localization to control the SREBP pathway. Cell. 2011;146:408-20 pubmed publisher
product information
Catalog Number :
32018
Product Name :
pLKO-puro FLAG SREBP2
article :
doi10.1016/j.cell.2011.06.034
id4571
pubmed_id21816276
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1-puro
backbone_mutationU6 replaced by CMV and FLAG added N-term to insert. 5->3': CMV-> FLAG -> sal I restriction site -> 1 base(to keep in frame) -> cDNA -> Not I restriction site
backbone_origin
backbone_size7100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5SalI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSREBP-2, SREBP2, SREBP2gc, bHLHd2, lop13, nuc
geneSrebf2
id20788
genbank_ids
mutation
nameSREBP2
shRNA_sequence
size
species
10090
Mus musculus
tags
locationN terminal on backbone
tagFLAG
resistance markers :
432
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA