This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+) Flag-His-ATM kd
catalog :
31986
citations: 10
Reference
Li S, Liu B, Tan M, Juillard F, Szymula A, xc1 lvarez x, et al. Kaposi's sarcoma herpesvirus exploits the DNA damage response to circularize its genome. Nucleic Acids Res. 2024;: pubmed publisher
Banerjee D, Langberg K, Abbas S, Odermatt E, Yerramothu P, Volaric M, et al. A non-canonical, interferon-independent signaling activity of cGAMP triggers DNA damage response signaling. Nat Commun. 2021;12:6207 pubmed publisher
Sarkar A, Gandhi V. Activation of ATM kinase by ROS generated during ionophore-induced mitophagy in human T and B cell malignancies. Mol Cell Biochem. 2021;476:417-423 pubmed publisher
Sarkar A, Stellrecht C, Vangapandu H, Ayres M, Kaipparettu B, Park J, et al. Ataxia telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma. Haematologica. 2020;: pubmed publisher
Gross K, Zhou W, Breindel J, Ouyang J, Jin D, Sokol E, et al. Loss of Slug Compromises DNA Damage Repair and Accelerates Stem Cell Aging in Mammary Epithelium. Cell Rep. 2019;28:394-407.e6 pubmed publisher
Chow H, Cheng A, Song X, Swerdel M, Hart R, Herrup K. ATM is activated by ATP depletion and modulates mitochondrial function through NRF1. J Cell Biol. 2019;218:909-928 pubmed publisher
Salzman D, Nakamura K, Nallur S, Dookwah M, Metheetrairut C, Slack F, et al. miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage. Nat Commun. 2016;7:10954 pubmed publisher
Mavuluri J, Beesetti S, Surabhi R, Kremerskothen J, Venkatraman G, Rayala S. Phosphorylation-Dependent Regulation of the DNA Damage Response of Adaptor Protein KIBRA in Cancer Cells. Mol Cell Biol. 2016;36:1354-65 pubmed publisher
Kubota S, Fukumoto Y, Ishibashi K, Soeda S, Kubota S, Yuki R, et al. Activation of the prereplication complex is blocked by mimosine through reactive oxygen species-activated ataxia telangiectasia mutated (ATM) protein without DNA damage. J Biol Chem. 2014;289:5730-46 pubmed publisher
Canman C, Lim D, Cimprich K, Taya Y, Tamai K, Sakaguchi K, et al. Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science. 1998;281:1677-9 pubmed
product information
Catalog Number :
31986
Product Name :
pcDNA3.1(+) Flag-His-ATM kd
article :
doi10.1126/science.281.5383.1677
id4564
pubmed_id9733515
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Do not send out as a spot on paper, send out in liquid form.
growth temp :
After transformation, grow for 2 days at 30 degrees. Ignore colonies that grow after 1 day. Let grow for a full 2 days. See protocol -Transformation of the ATM Plasmid
origin :
30
pi :
alt_names
ATI
ATA
ATDC
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XhoI-SalI ligation
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesAT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1
geneATM
id472
genbank_ids
NP_000042.3
mutation3'UTRf 9 nucl/D2870A N2875k. catalytically inactive.
nameATM kd
shRNA_sequence
size9171
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFlag
locationN terminal on backbone
tagHis
plasmid copy :
ATM cDNA generated by Dr's Yael Ziv and Yossi Shiloh and with this cDNA Dr. Dae Sik Lim/laboratory of Michael Kastan sub-cloned it
resistance markers :
915
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA