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product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+) Flag-His-ATM kd
catalog :
31986
citations: 10
Reference |
---|
Canman C, Lim D, Cimprich K, Taya Y, Tamai K, Sakaguchi K, et al. Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science. 1998;281:1677-9 pubmed
|
product information
Catalog Number :
31986
Product Name :
pcDNA3.1(+) Flag-His-ATM kd
article :
doi | 10.1126/science.281.5383.1677 |
id | 4564 |
pubmed_id | 9733515 |
bacterial resistance :
Ampicillin
cloning :
backbone | pcDNA3.1 | |
backbone_mutation | ||
backbone_origin | Invitrogen | |
backbone_size | 5428 | |
promoter | ||
sequencing_primer_3 | ||
sequencing_primer_5 | ||
vector_types |
|
growth notes :
Do not send out as a spot on paper, send out in liquid form.
growth temp :
After transformation, grow for 2 days at 30 degrees. Ignore colonies that grow after 1 day. Let grow for a full 2 days. See protocol -Transformation of the ATM Plasmid
origin :
30
pi :
|
plasmid copy :
ATM cDNA generated by Dr's Yael Ziv and Yossi Shiloh and with this cDNA Dr. Dae Sik Lim/laboratory of Michael Kastan sub-cloned it
resistance markers :
915 |
tags :
Unknown
terms :
Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments