This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1(+)Flag-His-ATM wt
catalog :
31985
citations: 29
Reference
Li S, Liu B, Tan M, Juillard F, Szymula A, xc1 lvarez x, et al. Kaposi's sarcoma herpesvirus exploits the DNA damage response to circularize its genome. Nucleic Acids Res. 2024;: pubmed publisher
Bodnar Wachtel M, Huber A, Gorry J, Hacot S, Burlet D, G xe9 rossier L, et al. Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stress. Life Sci Alliance. 2023;6: pubmed publisher
Bourseguin J, Cheng W, Talbot E, Hardy L, Lai J, Jeffries A, et al. Persistent DNA damage associated with ATM kinase deficiency promotes microglial dysfunction. Nucleic Acids Res. 2022;50:2700-2718 pubmed publisher
Loke J, Alim I, Yam S, Klugman S, Xia L, Gruber D, et al. Prediction of breast cancer risk based on flow variant analysis of circulating peripheral blood mononuclear cells. HGG Adv. 2022;3:100085 pubmed publisher
Zhang J, Wu Q, Zhu L, Xie S, Tu L, Yang Y, et al. SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer. Cancer Lett. 2022;524:268-283 pubmed publisher
Banerjee D, Langberg K, Abbas S, Odermatt E, Yerramothu P, Volaric M, et al. A non-canonical, interferon-independent signaling activity of cGAMP triggers DNA damage response signaling. Nat Commun. 2021;12:6207 pubmed publisher
Petsalaki E, Zachos G. An ATM-Chk2-INCENP pathway activates the abscission checkpoint. J Cell Biol. 2021;220: pubmed publisher
Sun H, Zhu A, Gao Y, Terajima H, Fei Q, Liu S, et al. Stabilization of ERK-Phosphorylated METTL3 by USP5 Increases m6A Methylation. Mol Cell. 2020;80:633-647.e7 pubmed publisher
Sarkar A, Gandhi V. Activation of ATM kinase by ROS generated during ionophore-induced mitophagy in human T and B cell malignancies. Mol Cell Biochem. 2021;476:417-423 pubmed publisher
Sarkar A, Stellrecht C, Vangapandu H, Ayres M, Kaipparettu B, Park J, et al. Ataxia telangiectasia mutated interacts with Parkin and induces mitophagy independent of kinase activity. Evidence from mantle cell lymphoma. Haematologica. 2020;: pubmed publisher
Zhao J, Nguyen L, Nguyen L, Dang X, Cao D, Khanal S, et al. ATM Deficiency Accelerates DNA Damage, Telomere Erosion, and Premature T Cell Aging in HIV-Infected Individuals on Antiretroviral Therapy. Front Immunol. 2019;10:2531 pubmed publisher
Chow H, Cheng A, Song X, Swerdel M, Hart R, Herrup K. ATM is activated by ATP depletion and modulates mitochondrial function through NRF1. J Cell Biol. 2019;218:909-928 pubmed publisher
Carranza D, Torres Rusillo S, Ceballos Pérez G, Blanco Jimenez E, Muñoz Lopez M, Garcia Perez J, et al. Reconstitution of the Ataxia-Telangiectasia Cellular Phenotype With Lentiviral Vectors. Front Immunol. 2018;9:2703 pubmed publisher
Sethy R, Rakesh R, Patne K, Arya V, Sharma T, Haokip D, et al. Regulation of ATM and ATR by SMARCAL1 and BRG1. Biochim Biophys Acta Gene Regul Mech. 2018;1861:1076-1092 pubmed publisher
Clift D, So C, McEwan W, James L, Schuh M. Acute and rapid degradation of endogenous proteins by Trim-Away. Nat Protoc. 2018;13:2149-2175 pubmed publisher
McBrayer S, Mayers J, DiNatale G, Shi D, Khanal J, Chakraborty A, et al. Transaminase Inhibition by 2-Hydroxyglutarate Impairs Glutamate Biosynthesis and Redox Homeostasis in Glioma. Cell. 2018;175:101-116.e25 pubmed publisher
Qian M, Liu Z, Peng L, Tang X, Meng F, Ao Y, et al. Boosting ATM activity alleviates aging and extends lifespan in a mouse model of progeria. elife. 2018;7: pubmed publisher
Zhao J, Dang X, Zhang P, Nguyen L, Cao D, Wang L, et al. Insufficiency of DNA repair enzyme ATM promotes naive CD4 T-cell loss in chronic hepatitis C virus infection. Cell Discov. 2018;4:16 pubmed publisher
Deng Y, Li F, He P, Yang Y, Yang J, Zhang Y, et al. Triptolide sensitizes breast cancer cells to Doxorubicin through the DNA damage response inhibition. Mol Carcinog. 2018;57:807-814 pubmed publisher
Lung R, Hau P, Yu K, Yip K, Tong J, Chak W, et al. EBV-encoded miRNAs target ATM-mediated response in nasopharyngeal carcinoma. J Pathol. 2018;244:394-407 pubmed publisher
Chen Z, Cao Z, Zhang W, Gu M, Zhou Z, Li B, et al. LRRK2 interacts with ATM and regulates Mdm2-p53 cell proliferation axis in response to genotoxic stress. Hum Mol Genet. 2017;26:4494-4505 pubmed publisher
Li C, Park S, Zhang X, Eisenberg L, Zhao H, Darzynkiewicz Z, et al. Nuclear Gene 33/Mig6 regulates the DNA damage response through an ATM serine/threonine kinase-dependent mechanism. J Biol Chem. 2017;292:16746-16759 pubmed publisher
Sastre Moreno G, Pryor J, Moreno Oñate M, Herrero Ruiz A, Cortés Ledesma F, Blanco L, et al. Regulation of human polλ by ATM-mediated phosphorylation during non-homologous end joining. DNA Repair (Amst). 2017;51:31-45 pubmed publisher
Salzman D, Nakamura K, Nallur S, Dookwah M, Metheetrairut C, Slack F, et al. miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage. Nat Commun. 2016;7:10954 pubmed publisher
Mavuluri J, Beesetti S, Surabhi R, Kremerskothen J, Venkatraman G, Rayala S. Phosphorylation-Dependent Regulation of the DNA Damage Response of Adaptor Protein KIBRA in Cancer Cells. Mol Cell Biol. 2016;36:1354-65 pubmed publisher
Zhang J, Tripathi D, Jing J, Alexander A, Kim J, Powell R, et al. ATM functions at the peroxisome to induce pexophagy in response to ROS. Nat Cell Biol. 2015;17:1259-1269 pubmed publisher
Mohanty S, Saha S, Md S Hossain D, Adhikary A, Mukherjee S, Manna A, et al. ROS-PIAS? cross talk channelizes ATM signaling from resistance to apoptosis during chemosensitization of resistant tumors. Cell Death Dis. 2014;5:e1021 pubmed publisher
Kubota S, Fukumoto Y, Ishibashi K, Soeda S, Kubota S, Yuki R, et al. Activation of the prereplication complex is blocked by mimosine through reactive oxygen species-activated ataxia telangiectasia mutated (ATM) protein without DNA damage. J Biol Chem. 2014;289:5730-46 pubmed publisher
Canman C, Lim D, Cimprich K, Taya Y, Tamai K, Sakaguchi K, et al. Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science. 1998;281:1677-9 pubmed
product information
Catalog Number :
31985
Product Name :
pcDNA3.1(+)Flag-His-ATM wt
article :
doi10.1126/science.281.5383.1677
id4564
pubmed_id9733515
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian expression of human ATM with Flag and His tags
growth temp :
After transformation, grow for 2 days at 30 degrees. Ignore colonies that grow after 1 day. Let grow for a full 2 days.
origin :
30
pi :
alt_names
ATI
ATA
ATDC
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XhoI-SalI Ligation
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesAT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1
geneATM
id472
genbank_ids
NP_000042.3/71902540
mutation3' UTR of 400 nucl.
nameATM wild-type
shRNA_sequence
size9581
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFlag
locationN terminal on backbone
tagHIS
plasmid copy :
ATM cDNA generated by Dr's Yael Ziv and Yossi Shiloh and with this cDNA Dr. Dae Sik Lim/laboratory of Michael Kastan sub-cloned it
resistance markers :
915
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA