This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
OCT4-2A-eGFP-PGK-Puro
catalog :
31938
citations: 22
Reference
Repina N, Johnson H, Bao X, Zimmermann J, Joy D, Bi S, et al. Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture. Development. 2023;150: pubmed publisher
Ueda Y, Nakamura T, Nie J, Solivais A, Hoffman J, Daye B, et al. Defining developmental trajectories of prosensory cells in human inner ear organoids at single-cell resolution. Development. 2023;150: pubmed publisher
Nie J, Ueda Y, Solivais A, Hashino E. CHD7 regulates otic lineage specification and hair cell differentiation in human inner ear organoids. Nat Commun. 2022;13:7053 pubmed publisher
Ma X, Cao J, Zhou Z, Lu Y, Li Q, Jin Y, et al. N6-methyladenosine modification-mediated mRNA metabolism is essential for human pancreatic lineage specification and islet organogenesis. Nat Commun. 2022;13:4148 pubmed publisher
Chang Y, Syahirah R, Oprescu S, Wang X, Jung J, Cooper S, et al. Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells. Biomaterials. 2022;285:121569 pubmed publisher
Vojnits K, Nakanishi M, Porras D, Kim Y, Feng Z, Golubeva D, et al. Developing CRISPR/Cas9-Mediated Fluorescent Reporter Human Pluripotent Stem-Cell Lines for High-Content Screening. Molecules. 2022;27: pubmed publisher
Lee H, Lee J, Park N, Dubey S, Kim T, Ruan K, et al. Multi-omic analysis of selectively vulnerable motor neuron subtypes implicates altered lipid metabolism in ALS. Nat Neurosci. 2021;24:1673-1685 pubmed publisher
Alexeeva V, Aydin I, Schaniel C, Stranahan A, D Souza S, Bieker J. A human H1-HBB11-GFP reporter embryonic stem cell line (WAe001-A-2) generated using TALEN-based genome editing. Stem Cell Res. 2020;45:101837 pubmed publisher
Randolph L, Bao X, Oddo M, Lian X. Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation. Sci Rep. 2019;9:16696 pubmed publisher
Bao X, Adil M, Muckom R, Zimmermann J, Tran A, Suhy N, et al. Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing. Stem Cells. 2019;37:1556-1566 pubmed publisher
Tsai S, Ghazizadeh Z, Wang H, Ortega F, Badieyan Z, Hsu Z, et al. A Human Embryonic Stem Cell Reporter Line for Monitoring Chemical-induced Cardiotoxicity. Cardiovasc Res. 2019;: pubmed publisher
Chen Z, Ren X, Xu X, Zhang X, Hui Y, Liu Z, et al. Genetic Engineering of Human Embryonic Stem Cells for Precise Cell Fate Tracing during Human Lineage Development. Stem Cell Reports. 2018;11:1257-1271 pubmed publisher
Mukherjee S, Zhang T, Lacko L, Tan L, Xiang J, Butler J, et al. Derivation and characterization of a UCP1 reporter human ES cell line. Stem Cell Res. 2018;30:12-21 pubmed publisher
Ma X, Chen X, Jin Y, Ge W, Wang W, Kong L, et al. Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells. Nat Commun. 2018;9:1303 pubmed publisher
Klann T, Crawford G, Reddy T, Gersbach C. Screening Regulatory Element Function with CRISPR/Cas9-based Epigenome Editing. Methods Mol Biol. 2018;1767:447-480 pubmed publisher
Bao X, Bhute V, Han T, Qian T, Lian X, Palecek S. Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells. Bioeng Transl Med. 2017;2:191-201 pubmed publisher
Cai L, Bai H, Mahairaki V, Gao Y, He C, Wen Y, et al. A Universal Approach to Correct Various HBB Gene Mutations in Human Stem Cells for Gene Therapy of Beta-Thalassemia and Sickle Cell Disease. Stem Cells Transl Med. 2018;7:87-97 pubmed publisher
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product information
Catalog Number :
31938
Product Name :
OCT4-2A-eGFP-PGK-Puro
article :
doi10.1038/nbt.1927
id4552
pubmed_id21738127
bacterial resistance :
Ampicillin
cloning :
backbonePCR2.1
backbone_mutation
backbone_origininvitrogen
backbone_size8012
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
targeting donor
growth notes :
Please note that the Puromycin selection cassette in this plasmid has a R166H mutation. This mutation has been observed in other plasmids and should not have any affect on the function.
growth strain :
Human OCT4 genomic targeting donor
origin :
37
pi :
alt_names
Pou5F1
cloning
clone_methodRestriction Enzyme
cloning_site_3-
cloning_site_5-
promoter
sequencing_primer_3M13
sequencing_primer_5M13
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
genbank_ids
mutation
nameOCT4
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tageGFP
resistance markers :
227
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA