This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTight-hASCL1-N174
catalog :
31876
citations: 5
Reference
Meder L, Florin A, Ozretić L, Nill M, Koker M, Meemboor S, et al. Notch1 Deficiency Induces Tumor Cell Accumulation Inside the Bronchiolar Lumen and Increases TAZ Expression in an Autochthonous Kras LSL-G12V Driven Lung Cancer Mouse Model. Pathol Oncol Res. 2021;27:596522 pubmed publisher
Song S, Ashok A, Williams D, Kaufman M, Duff K, Sproul A. Efficient Derivation of Excitatory and Inhibitory Neurons from Human Pluripotent Stem Cells Stably Expressing Direct Reprogramming Factors. Curr Protoc. 2021;1:e141 pubmed publisher
El Sehemy A, Selvadurai H, Ortín Martínez A, Pokrajac N, Mamatjan Y, Tachibana N, et al. Norrin mediates tumor-promoting and -suppressive effects in glioblastoma via Notch and Wnt. J Clin Invest. 2020;130:3069-3086 pubmed publisher
Meder L, König K, Ozretić L, Schultheis A, Ueckeroth F, Ade C, et al. NOTCH, ASCL1, p53 and RB alterations define an alternative pathway driving neuroendocrine and small cell lung carcinomas. Int J Cancer. 2016;138:927-38 pubmed publisher
Yoo A, Sun A, Li L, Shcheglovitov A, Portmann T, Li Y, et al. MicroRNA-mediated conversion of human fibroblasts to neurons. Nature. 2011;476:228-31 pubmed publisher
product information
Catalog Number :
31876
Product Name :
pTight-hASCL1-N174
article :
doi10.1038/nature10323
id4500
pubmed_id21753754
bacterial resistance :
Ampicillin
cloning :
backbonepTight-N174
backbone_mutation
backbone_originhome made/Crabtree lab
backbone_size7998
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
origin :
37
pi :
alt_names
ASCL1
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3gtggatgtggaatgtgtgcga
sequencing_primer_5atgtcgaggtaggcgtgtac
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesASH1, HASH1, MASH1, bHLHa46
geneASCL1
id429
genbank_ids
mutation
nameachaete-scute complex homolog 1
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
97
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA