This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRS316-GFP-COF1
catalog :
31843
citations: 1
| Reference |
|---|
Okreglak V, Drubin D. Cofilin recruitment and function during actin-mediated endocytosis dictated by actin nucleotide state. J Cell Biol. 2007;178:1251-64 pubmed
|
product information
Catalog Number :
31843
Product Name :
pRS316-GFP-COF1
article :
| doi | 10.1083/jcb.200703092 |
| id | 4523 |
| pubmed_id | 17875745 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pRS316 | |
| backbone_mutation | ||
| backbone_origin | ATCC | |
| backbone_size | 4887 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
The GFP-cofilin construct has S65T-GFP and a 12 amino acid linker (GHGTGSTGSGSS) inserted in between amino acids N74 and G75 of cofilin. Note that the GFP is NOT N-terminal. A missense mutation was detected by the lab in sequencing: converting the third glycine in the 5 GFP linker into an arginine. This was the construct used in the paper.
origin :
37
pi :
|
resistance markers :
| 1025 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments