pTRE-TIGHT-Cx43-eYFP | Addgene 31807 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTRE-TIGHT-Cx43-eYFP

catalog :

31807

citations: 3

Reference
Genet N, Genet G, Chavkin N, Paila U, Fang J, Vasavada H, et al. Connexin 43-mediated neurovascular interactions regulate neurogenesis in the adult brain subventricular zone. Cell Rep. 2023;42:112371 pubmed publisher
de Solis C, Ho A, Holehonnur R, Ploski J. The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. Front Mol Neurosci. 2016;9:70 pubmed publisher
Smyth J, Hong T, Gao D, Vogan J, Jensen B, Fong T, et al. Limited forward trafficking of connexin 43 reduces cell-cell coupling in stressed human and mouse myocardium. J Clin Invest. 2010;120:266-79 pubmed publisher

product information

Catalog Number :

31807

Product Name :

pTRE-TIGHT-Cx43-eYFP

article :

doi	10.1172/JCI39740
id	3838
pubmed_id	20038810

bacterial resistance :

Ampicillin

cloning :

backbone

pTRE-TIGHT

backbone_mutation

backbone_origin

Clontech

backbone_size

2600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Human Cx43 was initially cloned into pEYFP-N1 using the NheI and HindIII cut sites of this vector. Cx43-EYFP was then excised from pEYFP-N1 using NheI and NotI to insert the fusion protein encoding genes into pTRE-Tight, so importantly, some of the MCS from pEYFP-N1 remains in the final construct.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	NheI
promoter	P-TIGHT
sequencing_primer_3	EXFP-R
sequencing_primer_5	LNCX_primer
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
gene	GJA1
id	2697

genbank_ids

mutation

name

Cx43

shRNA_sequence

size

species

9606

Homo sapiens