This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pT3-EF1a-Bmi1
catalog :
31783
citations: 5
Reference
Tanaka A, Watanabe A, Nakano Y, Matsumoto M, Okazaki Y, Miyajima A. Reversible expansion of pancreatic islet progenitors derived from human induced pluripotent stem cells. Genes Cells. 2020;25:302-311 pubmed publisher
Li W, Yu X, Xia Z, Yu X, Xie L, Ma X, et al. Repression of Noxa by Bmi1 contributes to deguelin-induced apoptosis in non-small cell lung cancer cells. J Cell Mol Med. 2018;22:6213-6227 pubmed publisher
Patel N, Garikapati K, Pandita R, Singh D, Pandita T, Bhadra U, et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin. Sci Rep. 2017;7:4263 pubmed publisher
Zheng S, Hedl M, Abraham C. Twist1 and Twist2 Contribute to Cytokine Downregulation following Chronic NOD2 Stimulation of Human Macrophages through the Coordinated Regulation of Transcriptional Repressors and Activators. J Immunol. 2015;195:217-26 pubmed publisher
Xu C, Lee S, Ho C, Bommi P, Huang S, Cheung S, et al. Bmi1 functions as an oncogene independent of Ink4A/Arf repression in hepatic carcinogenesis. Mol Cancer Res. 2009;7:1937-45 pubmed publisher
product information
Catalog Number :
31783
Product Name :
pT3-EF1a-Bmi1
article :
doi10.1158/1541-7786.MCR-09-0333
id4501
pubmed_id19934271
bacterial resistance :
Ampicillin
cloning :
backbonepT3-EF1a
backbone_mutation
backbone_origin
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid is in the pT3-EF1a vector which contains loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid cannot not used with Cre for in vivo studies.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterEF1alpha
sequencing_primer_3
sequencing_primer_5EF-1a-Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1
geneBMI1
id648
genbank_ids
mutation
nameBmi1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA
resistance markers :
1021
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA