This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBMN FKBP(DD)-YFP
catalog :
31763
citations: 5
Reference
Martin M, Sandhu P, Kumar R, Buchkovich N. The Immune-Specific E3 Ubiquitin Ligase MARCH1 Is Upregulated during Human Cytomegalovirus Infection to Regulate Iron Levels. J Virol. 2022;96:e0180621 pubmed publisher
Escobar T, Oksuz O, SaldaƱa Meyer R, Descostes N, Bonasio R, Reinberg D. Active and Repressed Chromatin Domains Exhibit Distinct Nucleosome Segregation during DNA Replication. Cell. 2019;179:953-963.e11 pubmed publisher
Kogenaru M, Isalan M. Drug-Inducible Control of Lethality Genes: A Low Background Destabilizing Domain Architecture Applied to the Gal4-UAS System in Drosophila. ACS Synth Biol. 2018;7:1496-1506 pubmed publisher
Qi Z, Wilkinson M, Chen X, Sankararaman S, Mayhew D, Mitra R. An optimized, broadly applicable piggyBac transposon induction system. Nucleic Acids Res. 2017;45:e55 pubmed publisher
Banaszynski L, Chen L, Maynard Smith L, Ooi A, Wandless T. A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell. 2006;126:995-1004 pubmed
product information
Catalog Number :
31763
Product Name :
pBMN FKBP(DD)-YFP
article :
doi10.1016/j.cell.2006.07.025
id4495
pubmed_id16959577
bacterial resistance :
Ampicillin
cloning :
backbonepBMN
backbone_mutation
backbone_originGarry Nolan
backbone_size6943
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
growth temp :
Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C
origin :
30
pi :
alt_names
YFP and FKBP12 (destable domain)
YFP fused with FK506 binding protein 1A( destable domain)
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterLTR
sequencing_primer_3
sequencing_primer_5pBMN specific
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE
geneFKBP1A
id2280
genbank_ids
NM_000801
mutationF36V, L106P on FKBP12
nameFKBP(DD)-YFP-HA
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagYFP
locationC terminal on backbone
tagHA
resistance markers :
720
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA