pRK5F-ALK5-HA(ecto) | Addgene 31720 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRK5F-ALK5-HA(ecto)

catalog :

31720

citations: 1

Reference
Murillo Garzón V, Gorroño Etxebarria I, Akerfelt M, Puustinen M, Sistonen L, Nees M, et al. Frizzled-8 integrates Wnt-11 and transforming growth factor-β signaling in prostate cancer. Nat Commun. 2018;9:1747 pubmed publisher

product information

Catalog Number :

31720

Product Name :

pRK5F-ALK5-HA(ecto)

article :

doi
id	4486
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5

backbone_mutation

backbone_origin

backbone_size

4716

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This construct is beginning from a Signal Peptide of human TbRI/ALK5, followed by a HA tag, the extracellular cDNA sequence of human TbRI/ALK, and ended by a Flag tag. This vector to produces a secreted TbRI/ALK5 extracellular form, with two individual tags on each side.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	EcoRI
promoter
sequencing_primer_3	M13_puc_Fwd
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, TBR-i, TBRI, TGFR-1, tbetaR-I
gene	TGFBR1
id	7046

genbank_ids

mutation

name

ALK5

shRNA_sequence

size

species

9606

Homo sapiens