This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-PICK
catalog :
31613
citations: 2
Reference
Soskis M, Ho H, Bloodgood B, Robichaux M, Malik A, Ataman B, et al. A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development. Nat Neurosci. 2012;15:1645-54 pubmed publisher
Citri A, Bhattacharyya S, Ma C, Morishita W, Fang S, Rizo J, et al. Calcium binding to PICK1 is essential for the intracellular retention of AMPA receptors underlying long-term depression. J Neurosci. 2010;30:16437-52 pubmed publisher
product information
Catalog Number :
31613
Product Name :
GFP-PICK
article :
doi10.1523/JNEUROSCI.4478-10.2010
id4465
pubmed_id21147983
bacterial resistance :
Ampicillin
cloning :
backboneFUGW
backbone_mutation
backbone_originDavid Baltimore (Caltech)
backbone_size9941
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
This is the replacement construct which expresses an shRNA to knockdown the endogenous protein and a recombinant GFP-tagged, shRNA-resistant protein, to replace the endogenous PICK1. shRNA sequence: CTATGAGTACCGCCTTATCCT
growth temp :
Stbl3
origin :
37
pi :
alt_names
PICK1
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPrkcabp
genePick1
id84591
genbank_ids
mutation
namePICK1 (shRNA insensitive) +shRNA towards PICK1
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagGFP
locationN terminal on insert
tagHA
locationN terminal on backbone
tagPICK1 shRNA
resistance markers :
1006
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA