pLiLucB | Addgene 31596 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLiLucB

catalog :

31596

citations: 1

Reference
Woodard L, Keravala A, Jung W, Wapinski O, Yang Q, Felsher D, et al. Impact of hydrodynamic injection and phiC31 integrase on tumor latency in a mouse model of MYC-induced hepatocellular carcinoma. PLoS ONE. 2010;5:e11367 pubmed publisher

product information

Catalog Number :

31596

Product Name :

pLiLucB

article :

doi	10.1371/journal.pone.0011367
id	4458
pubmed_id	20614008

bacterial resistance :

Kanamycin

cloning :

backbone

pNBL2

backbone_mutation

backbone_origin

Calos Lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

As described in the methods: pLiLucB is a liver-specific, luciferase-expressing attB donor plasmid that was constructed by digesting pNBL2 with XhoI to remove the CMV promoter and digesting pVFB with EcoRI to obtain the human alpha-1 antitrypsin promoter with an apolipoprotein enhancer. The ends of both fragments were made blunt by filling in with Klenow polymerase, and the pNBL2 backbone was treated with phosphatase. The construct was ligated using T4 ligase and checked by XcmI digestion and sequencing of the promoter region.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	Luc_N_Rev
sequencing_primer_5	hAAT-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF
gene	SERPINA1
id	5265

genbank_ids

mutation

name

alpha-1 antitrypsin promoter with an apolipoprotein enhancer

shRNA_sequence

size

species

9606

Homo sapiens