This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pQTEV-ANP32A
catalog :
31563
citations: 3
Reference
Camacho Zarco A, Yu L, Krischuns T, Dedeoglu S, Maurin D, Bouvignies G, et al. Multivalent Dynamic Colocalization of Avian Influenza Polymerase and Nucleoprotein by Intrinsically Disordered ANP32A Reveals the Molecular Basis of Human Adaptation. J Am Chem Soc. 2023;145:20985-21001 pubmed publisher
Camacho Zarco A, Kalayil S, Maurin D, Salvi N, Delaforge E, Milles S, et al. Molecular basis of host-adaptation interactions between influenza virus polymerase PB2 subunit and ANP32A. Nat Commun. 2020;11:3656 pubmed publisher
Büssow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, et al. Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones. Microb Cell Fact. 2005;4:21 pubmed
product information
Catalog Number :
31563
Product Name :
pQTEV-ANP32A
article :
doi10.1186/1475-2859-4-21
id4398
pubmed_id15998469
bacterial resistance :
Ampicillin
cloning :
backbonepQTEV
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence.
inserts :
Rosetta
origin :
37
pi :
alt_names
PSFEp250D092
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoter
sequencing_primer_3GGCAACCGAGCGTTCTGAAC
sequencing_primer_5TGAGCGGATAACAATTTCACACAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC15orf1, HPPCn, I1PP2A, LANP, MAPM, PHAP1, PHAPI, PP32
geneANP32A
id8125
genbank_ids
DQ000553
mutation
nameacidic (leucine-rich) nuclear phosphoprotein 32 family, member A
shRNA_sequence
size822
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
locationN terminal on backbone
tagTEV
resistance markers :
58
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA