This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pQTEV-TPT1
catalog :
31558
citations: 1
Reference
Büssow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, et al. Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones. Microb Cell Fact. 2005;4:21 pubmed
product information
Catalog Number :
31558
Product Name :
pQTEV-TPT1
article :
doi10.1186/1475-2859-4-21
id4398
pubmed_id15998469
bacterial resistance :
Ampicillin
cloning :
backbonepQTEV
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence. Please note that Addgene's quality control sequence shows a small deletion in the vector region downstream of insert; the deletion does not affect any features in this construct.
inserts :
Rosetta
origin :
37
pi :
alt_names
PSFEp250G011
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoter
sequencing_primer_3GGCAACCGAGCGTTCTGAAC
sequencing_primer_5TGAGCGGATAACAATTTCACACAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHRF, TCTP, p02, p23
geneTPT1
id7178
genbank_ids
DQ000539
mutation
nametumor protein, translationally-controlled 1
shRNA_sequence
size591
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
locationN terminal on backbone
tagTEV
resistance markers :
58
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA