This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Reduced Expression GFP beta actin
catalog :
31502
citations: 7
Reference
Mahlandt E, Kreider Letterman G, Chertkova A, Garcia Mata R, Goedhart J. Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity. J Cell Sci. 2023;136: pubmed publisher
Quintanilla M, Patel H, Wu H, Sochacki K, Akamatsu M, Rotty J, et al. Local Monomer Levels and Established Filaments Potentiate Non-Muscle Myosin 2 Assembly. bioRxiv. 2023;: pubmed publisher
Mahlandt E, Arts J, van der Meer W, van der Linden F, Tol S, van Buul J, et al. Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor. J Cell Sci. 2021;: pubmed publisher
Zhang Y, Cao F, Zhou Y, Feng Z, Sit B, Krendel M, et al. Tail domains of myosin-1e regulate phosphatidylinositol signaling and F-actin polymerization at the ventral layer of podosomes. Mol Biol Cell. 2019;30:622-635 pubmed publisher
Spiliotis E, Karasmanis E, Dolat L. Fluorescence microscopy of actin- and microtubule-associated septins in mammalian cells. Methods Cell Biol. 2016;136:243-68 pubmed publisher
Klawitter S, Fuchs N, Upton K, Muñoz Lopez M, Shukla R, Wang J, et al. Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells. Nat Commun. 2016;7:10286 pubmed publisher
Watanabe N, Mitchison T. Single-molecule speckle analysis of actin filament turnover in lamellipodia. Science. 2002;295:1083-6 pubmed
product information
Catalog Number :
31502
Product Name :
Reduced Expression GFP beta actin
article :
doi10.1126/science.1067470
id4446
pubmed_id11834838
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4250
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoter
sequencing_primer_3
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
NM_001101
mutation91-544 of enhancer region of CMV promoter deleted
namebeta actin
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
208
1002
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA