pSLIK sh human Rb 1534 hyg | Addgene 31500 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSLIK sh human Rb 1534 hyg

catalog :

31500

citations: 2

Reference
Chen H, Gardner E, Shah Y, Zhang K, Thakur A, Zhang C, et al. FORMATION OF MALIGNANT, METASTATIC SMALL CELL LUNG CANCERS THROUGH OVERPRODUCTION OF cMYC PROTEIN IN TP53 AND RB1 DEPLETED PULMONARY NEUROENDOCRINE CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS. bioRxiv. 2023;: pubmed publisher
Scully K, Lahmy R, Signaevskaia L, Sasik R, Medal R, Kim H, et al. E47 Governs the MYC-CDKN1B/p27KIP1-RB Network to Growth Arrest PDA Cells Independent of CDKN2A/p16INK4A and Wild-Type p53. Cell Mol Gastroenterol Hepatol. 2018;6:181-198 pubmed publisher

product information

Catalog Number :

31500

Product Name :

pSLIK sh human Rb 1534 hyg

article :

doi
id	4444
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pSLIK-EGFP

backbone_mutation

backbone_origin

backbone_size

13700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

growth notes :

The shRNA is downstream of the EGFP GGATAATCGTT pSLIK vector from Ian Frasier
AGCGAAACGATTATCCATTCAAATAGTGAAGCCACAGAT
GTATTTGAAT
The whole sequence for the shRNA including the loop should be from 3872-3931 in the vector:

growth temp :

Stbl3

origin :

pi :

alt_names

RB1

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	EGFP-C
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
gene	RB1
id	5925

genbank_ids

mutation

The target sequence for shRB 1534 is GAACGATTATCCATTCAAA

name

RB shRNA

shRNA_sequence

size

species

9606

Homo sapiens