This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLS1
catalog :
31490
citations: 3
Reference
Groseclose T, Rondon R, Herde Z, Aldrete C, Wilson C. Engineered systems of inducible anti-repressors for the next generation of biological programming. Nat Commun. 2020;11:4440 pubmed publisher
Tungtur S, Schwingen K, Riepe J, Weeramange C, Swint Kruse L. Homolog comparisons further reconcile in vitro and in vivo correlations of protein activities by revealing over-looked physiological factors. Protein Sci. 2019;28:1806-1818 pubmed publisher
Swint Kruse L, Elam C, Lin J, Wycuff D, Shive Matthews K. Plasticity of quaternary structure: twenty-two ways to form a LacI dimer. Protein Sci. 2001;10:262-76 pubmed
product information
Catalog Number :
31490
Product Name :
pLS1
article :
doi10.1110/ps.35801
id4440
pubmed_id11266612
bacterial resistance :
Ampicillin
cloning :
backbonepEMBL9'
backbone_mutation
backbone_origin
backbone_size4540
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Parent plasmid was pEMBL9' with insert of extended LacI sequence from pIQ (Hare & Sadler, Gene 3 (1978) 269-278. For expression: E. coli BLIM cells (or any E. coli WITHOUT lactose repressor in the genome or accompanying plasmid)
origin :
37
pi :
alt_names
LacI
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5EcoR1
promoter
sequencing_primer_3CTGGGCGTGGAGCATCTG
sequencing_primer_5CCAGCCAGCCAGACGCAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0345, ECK0342
genelacI
id945007
genbank_ids
EG10525
mutation
namelactose repressor protein
shRNA_sequence
size3000
species
tags
plasmid copy :
Original LacI sequence from pIQ obtained from Joan Betz (see reference below to Hare and Sadler for generation of the pIQ
resistance markers :
1004
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA