This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pINT-PuroDDHNF4a2
catalog :
31443
citations: 1
Reference
Waldner C, Rempel O, Schütte F, Yanik M, Solomentsew N, Ryffel G. Double conditional human embryonic kidney cell line based on FLP and ?C31 mediated transgene integration. BMC Res Notes. 2011;4:420 pubmed publisher
product information
Catalog Number :
31443
Product Name :
pINT-PuroDDHNF4a2
article :
doi10.1186/1756-0500-4-420
id5581
pubmed_id22008483
bacterial resistance :
Ampicillin
cloning :
backbonepCSGFP
backbone_mutation
backbone_origin
backbone_size3500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
phiC31/attB
growth notes :
Integration vector with attB site for phiC31 mediated integration of HNF4A2 into attP site using puromycin selection. HNF4A2 linked to destabilization domain (FKBP L106P) allowing shield inducibility.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14
geneHNF4A
id3172
genbank_ids
mutation
nameHNF4A
shRNA_sequence
size3000
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFKBP L106P
resistance markers :
971
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA