This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hPDI1_18-508_WT_pFLAG-CMV1
catalog :
31382
citations: 3
Reference
Culver J, Mariappan M. Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins. J Cell Biol. 2021;220: pubmed publisher
Luo Y, Jacobs E, Greco T, Mohammed K, Tong T, Keegan S, et al. HIV-host interactome revealed directly from infected cells. Nat Microbiol. 2016;1:16068 pubmed
Zito E, Melo E, Yang Y, Wahlander Ã, Neubert T, Ron D. Oxidative protein folding by an endoplasmic reticulum-localized peroxiredoxin. Mol Cell. 2010;40:787-97 pubmed publisher
product information
Catalog Number :
31382
Product Name :
hPDI1_18-508_WT_pFLAG-CMV1
article :
doi10.1016/j.molcel.2010.11.010
id4417
pubmed_id21145486
bacterial resistance :
Ampicillin
cloning :
backbonepFLAG_CMV1
backbone_mutationnone
backbone_originSigma
backbone_size4732
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
prolyl 4-hydroxylase, beta polypeptide
PDIA1
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5HindIII
promoter
sequencing_primer_3FLAG CMV 2AS
sequencing_primer_5CMVF
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB
geneP4HB
id5034
genbank_ids
mutation
namehPDI1
shRNA_sequence
size1514
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG M2
plasmid copy :
A bacterial expression vector for human PDI1 (hPDI1(18-508)pTrcHis-A) was a gift of Professor Colin Thorpe for U Delaware
resistance markers :
421
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA