This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pQTEV-STMN1
catalog :
31326
citations: 3
Reference
Moreno Marmol T, Ledesma Terrón M, Tabanera N, Martín Bermejo M, Cardozo M, Cavodeassi F, et al. Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis. elife. 2021;10: pubmed publisher
Taverna E, Mora Bermúdez F, Strzyz P, Florio M, Icha J, Haffner C, et al. Non-canonical features of the Golgi apparatus in bipolar epithelial neural stem cells. Sci Rep. 2016;6:21206 pubmed publisher
Büssow K, Scheich C, Sievert V, Harttig U, Schultz J, Simon B, et al. Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones. Microb Cell Fact. 2005;4:21 pubmed
product information
Catalog Number :
31326
Product Name :
pQTEV-STMN1
article :
doi10.1186/1475-2859-4-21
id4398
pubmed_id15998469
bacterial resistance :
Ampicillin
cloning :
backbonepQTEV
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
The vector pQTEV is derived from Qiagen pQE-2 and contains an inactive chloramphenicol resistance gene sequence. The Rosetta bacterial strain contains an additional plasmid. The second plasmid, pRARE, provides rare tRNAs for overexpression of recombinant proteins. It has 4694 bp and is chloramphenicol resistence. Please note that Addgene's quality control sequence shows a small deletion in the vector region downstream of insert; the deletion does not affect any features in this construct.
inserts :
Rosetta
origin :
37
pi :
alt_names
PSFEp250F072
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoter
sequencing_primer_3GGCAACCGAGCGTTCTGAAC
sequencing_primer_5TGAGCGGATAACAATTTCACACAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC1orf215, LAP18, Lag, OP18, PP17, PP19, PR22, SMN
geneSTMN1
id3925
genbank_ids
DQ000556
mutation
namestathmin 1/oncoprotein 18
shRNA_sequence
size522
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
locationN terminal on backbone
tagTEV
resistance markers :
58
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA