pAdEasy-Msln-iCre-HA-Flag | Addgene 31305 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAdEasy-Msln-iCre-HA-Flag

catalog :

31305

citations: 3

Reference
He X, Wang L, Riedel H, Wang K, Yang Y, Dinu C, et al. Mesothelin promotes epithelial-to-mesenchymal transition and tumorigenicity of human lung cancer and mesothelioma cells. Mol Cancer. 2017;16:63 pubmed publisher
He X, Despeaux E, Stueckle T, Chi A, Castranova V, Dinu C, et al. Role of mesothelin in carbon nanotube-induced carcinogenic transformation of human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2016;311:L538-49 pubmed publisher
Zhou B, Honor L, He H, Ma Q, Oh J, Butterfield C, et al. Adult mouse epicardium modulates myocardial injury by secreting paracrine factors. J Clin Invest. 2011;121:1894-904 pubmed publisher

product information

Catalog Number :

31305

Product Name :

pAdEasy-Msln-iCre-HA-Flag

article :

doi	10.1172/JCI45529
id	4401
pubmed_id	21505261

bacterial resistance :

Kanamycin

cloning :

backbone

pAdEasy

backbone_mutation

backbone_origin

Stratagene

backbone_size

33000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Adenoviral

growth notes :

Msln Promoter sequence provided by author (see above link) Please note that Addgene was unable to sequence verify this plasmid. The depositing laboratory PCR amplified two regions of this plasmid using the following primer pairs and sequenced the resulting PCR products to confirm the plasmid. Msln-1F: TCATTTGTTCCCTTTGACGGC Msln-1R: CTCTCCCCAGCATCCACATTC Expect 987 nt product Msln-2F: TTGCCTACAACACCCTGCTGCG Msln-2R: GCTCACAATGCTTCCATCAAACG Expect 792 nt product

origin :

pi :

alt_names

Msln

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	CGCGTTTTCATCATTGTC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MPF, SMRP
gene	MSLN
id	10232

genbank_ids

mutation

name

Mesothelin

shRNA_sequence

size

1850

species

9606

Homo sapiens