This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-4T2 Parkin RBR
catalog :
31255
citations: 2
Reference
Tan K, Haelterman N, Kwartler C, Regalado E, Lee P, Nagarkar Jaiswal S, et al. Ari-1 Regulates Myonuclear Organization Together with Parkin and Is Associated with Aortic Aneurysms. Dev Cell. 2018;45:226-244.e8 pubmed publisher
Wenzel D, Lissounov A, Brzovic P, Klevit R. UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids. Nature. 2011;474:105-8 pubmed publisher
product information
Catalog Number :
31255
Product Name :
pGEX-4T2 Parkin RBR
article :
doi10.1038/nature09966
id4385
pubmed_id21532592
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T2
backbone_mutation
backbone_origin
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Joch M. et al, (2007) Parkin-mediated monoubiquitination of the PDZ protein PICK1 regulates the activity of acid-sensing ion channels. Mol. Biol. Cell 18: 3105-18
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1
cloning_site_5Sal1
promoter
sequencing_primer_3
sequencing_primer_5GST sequencing primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPark, Park2
genePrkn
id56816
genbank_ids
mutationRBR domain, residues 217-465
nameParkin
shRNA_sequence
size750
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagGST
plasmid copy :
The lab of Edward Fon
resistance markers :
261
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA