This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFLAG-XBP1u-FLuc
catalog :
31239
citations: 6
Reference
Kranz P, Sänger C, Wolf A, Baumann J, Metzen E, Baumann M, et al. Tumor cells rely on the thiol oxidoreductase PDI for PERK signaling in order to survive ER stress. Sci Rep. 2020;10:15299 pubmed publisher
Chen S, Henderson A, Petriello M, Romano K, Gearing M, Miao J, et al. Trimethylamine N-Oxide Binds and Activates PERK to Promote Metabolic Dysfunction. Cell Metab. 2019;30:1141-1151.e5 pubmed publisher
Chen D, Xiang Q, Nicholas J. Human Herpesvirus 8 Interleukin-6 Interacts with Calnexin Cycle Components and Promotes Protein Folding. J Virol. 2017;91: pubmed publisher
Kranz P, Neumann F, Wolf A, Classen F, Pompsch M, Ocklenburg T, et al. PDI is an essential redox-sensitive activator of PERK during the unfolded protein response (UPR). Cell Death Dis. 2017;8:e2986 pubmed publisher
Hussmann M, Janke K, Kranz P, Neumann F, Mersch E, Baumann M, et al. Depletion of the thiol oxidoreductase ERp57 in tumor cells inhibits proliferation and increases sensitivity to ionizing radiation and chemotherapeutics. Oncotarget. 2015;6:39247-61 pubmed publisher
Jheng J, Lau K, Tang W, Wu M, Horng J. Endoplasmic reticulum stress is induced and modulated by enterovirus 71. Cell Microbiol. 2010;12:796-813 pubmed publisher
product information
Catalog Number :
31239
Product Name :
pFLAG-XBP1u-FLuc
article :
doi10.1111/j.1462-5822.2010.01434.x
id4378
pubmed_id20070307
bacterial resistance :
Ampicillin
cloning :
backbonepTriEx-4
backbone_mutationRenilla luciferase and the 5 untranslated region (UTR) containing IRES of EV71 is included upstream of the FLAG-XBP1u-FLuc insert.
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Insect Expression
growth notes :
EV71 5 UTR between Renilla and firefly luciferase.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho1
cloning_site_5Xho1
promoter
sequencing_primer_3TriExDOWN primer
sequencing_primer_5TriExUP primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesTREB-5, TREB5, XBP-1, XBP2
geneXBP1
id7494
genbank_ids
mutation
nameFLAG-XBP1u-FLuc
shRNA_sequence
size1700
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
locationC terminal on insert
tagFluc
resistance markers :
978
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA