This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti6/V5-DEST-HMGB1
catalog :
31208
citations: 2
Reference
Wei J, Alfajaro M, DeWeirdt P, Hanna R, Lu Culligan W, Cai W, et al. Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Cell. 2021;184:76-91.e13 pubmed publisher
Scott K, Nogueira C, Heffernan T, van Doorn R, Dhakal S, Hanna J, et al. Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Cancer Cell. 2011;20:92-103 pubmed publisher
product information
Catalog Number :
31208
Product Name :
pLenti6/V5-DEST-HMGB1
article :
doi10.1016/j.ccr.2011.05.025
id5143
pubmed_id21741599
bacterial resistance :
Ampicillin
cloning :
backbonepLenti6/V5-DEST
backbone_mutation
backbone_originInvitrogen
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth temp :
Grow in STBL3 bacteria (Invitrogen) at lower temps (~30C) to prevent recombination and increase plasmid production.
origin :
30
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3attR2
cloning_site_5attR1
promoter
sequencing_primer_3V5 Reverse
sequencing_primer_5CMV Forward
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesHMG-1, HMG1, HMG3, SBP-1
geneHMGB1
id3146
genbank_ids
BC003378
mutation
nameHMGB1
shRNA_sequence
size648
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagV5
plasmid copy :
The entry clone used to create this plasmid was obatined from the human ORFeome (v5.1) collection made available by the Center for Cancer Systems Biology at the Dana-Farber Cancer Institute in Boston, MA.
resistance markers :
78
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA