pDONR223-MEKDD | Addgene 31202 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223-MEKDD

catalog :

31202

citations: 5

Reference
Xu Y, Wang X, Chen Y, Chen S, Yang X, Sun Y, et al. Integration of Receptor Tyrosine Kinases Determines Sensitivity to PI3K?-selective Inhibitors in Breast Cancer. Theranostics. 2017;7:974-986 pubmed publisher
Arteaga M, Shang N, Ding X, Yong S, Cotler S, Denning M, et al. Inhibition of SIRT2 suppresses hepatic fibrosis. Am J Physiol Gastrointest Liver Physiol. 2016;310:G1155-68 pubmed publisher
Wang X, Zhang X, Li B, Zhai X, Yang Z, Ding L, et al. Simultaneous targeting of PI3KÎ´ and a PI3KÎ´-dependent MEK1/2-Erk1/2 pathway for therapy in pediatric B-cell acute lymphoblastic leukemia. Oncotarget. 2014;5:10732-44 pubmed
Zinn R, Gardner E, Marchionni L, Murphy S, Dobromilskaya I, Hann C, et al. ERK phosphorylation is predictive of resistance to IGF-1R inhibition in small cell lung cancer. Mol Cancer Ther. 2013;12:1131-9 pubmed publisher
Yang X, Boehm J, Yang X, Salehi Ashtiani K, Hao T, Shen Y, et al. A public genome-scale lentiviral expression library of human ORFs. Nat Methods. 2011;8:659-61 pubmed publisher

product information

Catalog Number :

31202

Product Name :

pDONR223-MEKDD

article :

doi	10.1038/nmeth.1638
id	4432
pubmed_id	21706014

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5005

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Donor vector

growth notes :

The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections. A set of 4 custom lentiviral vectors is available (http://www.addgene.org/25895, http://www.addgene.org/25896, http://www.addgene.org/25897, http://www.addgene.org/25890) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
gene	MAP2K1
id	5604

genbank_ids

mutation

S218D S222D

name

MAPKK1

shRNA_sequence

size

1200

species

9606

Homo sapiens