pDONR223-H-RAS V12 | Addgene 31201 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223-H-RAS V12

catalog :

31201

citations: 2

Reference
Xu Y, Wang X, Chen Y, Chen S, Yang X, Sun Y, et al. Integration of Receptor Tyrosine Kinases Determines Sensitivity to PI3K?-selective Inhibitors in Breast Cancer. Theranostics. 2017;7:974-986 pubmed publisher
Yang X, Boehm J, Yang X, Salehi Ashtiani K, Hao T, Shen Y, et al. A public genome-scale lentiviral expression library of human ORFs. Nat Methods. 2011;8:659-61 pubmed publisher

product information

Catalog Number :

31201

Product Name :

pDONR223-H-RAS V12

article :

doi	10.1038/nmeth.1638
id	4432
pubmed_id	21706014

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5005

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Donor vector

growth notes :

Do NOT use this plasmid directly for transfections, you will need to recombine it into a destination vector. A set of 4 custom lentiviral vectors is available (http://www.addgene.org/25895, http://www.addgene.org/25896, http://www.addgene.org/25897, http://www.addgene.org/25890) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

genbank_ids

mutation

G12V constitutively active

name

H-Ras

shRNA_sequence

size

700

species

9606

Homo sapiens