pDONR223-K-RAS V12 | Addgene 31200 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDONR223-K-RAS V12

catalog :

31200

citations: 3

Reference
Nabet B, Roberts J, Buckley D, Paulk J, Dastjerdi S, Yang A, et al. The dTAG system for immediate and target-specific protein degradation. Nat Chem Biol. 2018;14:431-441 pubmed publisher
Popovic M, Rensen de Leeuw M, Rehmann H. Selectivity of CDC25 homology domain-containing guanine nucleotide exchange factors. J Mol Biol. 2013;425:2782-94 pubmed publisher
Yang X, Boehm J, Yang X, Salehi Ashtiani K, Hao T, Shen Y, et al. A public genome-scale lentiviral expression library of human ORFs. Nat Methods. 2011;8:659-61 pubmed publisher

product information

Catalog Number :

31200

Product Name :

pDONR223-K-RAS V12

article :

doi	10.1038/nmeth.1638
id	4432
pubmed_id	21706014

bacterial resistance :

Spectinomycin

cloning :

backbone

pDONR223

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5005

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Gateway Donor vector

growth notes :

Do NOT use this plasmid directly for transfections, you will need to recombine it into a destination vector. A set of 4 custom lentiviral vectors is available (http://www.addgene.org/25895, http://www.addgene.org/25896, http://www.addgene.org/25897, http://www.addgene.org/25890) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13rev
sequencing_primer_5	M13pUC-fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2
gene	KRAS
id	3845

genbank_ids

mutation

G12V constitutively active

name

K-Ras

shRNA_sequence

size

570

species

9606

Homo sapiens