This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHYRS52
catalog :
31122
citations: 10
Reference
Nelson B, Soper N, Lupoli T. Bacterial J-Domains with C-Terminal Tags Contact the Substrate Binding Domain of DnaK and Sequester Chaperone Activity. Chembiochem. 2023;24:e202300261 pubmed publisher
Richards A, Yawson G, Nelson B, Lupoli T. Complementary protocols to evaluate inhibitors against the DnaK chaperone network. STAR Protoc. 2022;3:101381 pubmed publisher
Braffman N, Ruskoski T, Davis K, Glasser N, Johnson C, Okafor C, et al. Structural basis for an unprecedented enzymatic alkylation in cylindrocyclophane biosynthesis. elife. 2022;11: pubmed publisher
Yin Y, Feng X, Yu H, Fay A, Kovach A, Glickman M, et al. Structural basis for aggregate dissolution and refolding by the Mycobacterium tuberculosis ClpB-DnaK bi-chaperone system. Cell Rep. 2021;35:109166 pubmed publisher
Maddi E, Natesh R. Optimization strategies for expression and purification of soluble N-terminal domain of human centriolar protein SAS-6 in Escherichia coli. Protein Expr Purif. 2021;183:105856 pubmed publisher
Harnagel A, Lopez Quezada L, Park S, Baranowski C, Kieser K, Jiang X, et al. Nonredundant functions of Mycobacterium tuberculosis chaperones promote survival under stress. Mol Microbiol. 2020;: pubmed publisher
Lopez Quezada L, Smith R, Lupoli T, Edoo Z, Li X, Gold B, et al. Activity-Based Protein Profiling Reveals That Cephalosporins Selectively Active on Non-replicating Mycobacterium tuberculosis Bind Multiple Protein Families and Spare Peptidoglycan Transpeptidases. Front Microbiol. 2020;11:1248 pubmed publisher
Loving H, Underbakke E. Conformational Dynamics of FERM-Mediated Autoinhibition in Pyk2 Tyrosine Kinase. Biochemistry. 2019;58:3767-3776 pubmed publisher
Lupoli T, Fay A, Adura C, Glickman M, Nathan C. Reconstitution of a Mycobacterium tuberculosis proteostasis network highlights essential cofactor interactions with chaperone DnaK. Proc Natl Acad Sci U S A. 2016;113:E7947-E7956 pubmed
Muona M, Aranko A, Iwai H. Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing. Chembiochem. 2008;9:2958-61 pubmed publisher
product information
Catalog Number :
31122
Product Name :
pHYRS52
article :
doi10.1002/cbic.200800604
id2420
pubmed_id19031436
bacterial resistance :
Ampicillin
cloning :
backbonepRsetA
backbone_mutation
backbone_origininvitrogen
backbone_size2894
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Mossessova E, Lima CD. Mol Cell. 2000 May;5(5):865-76. Note: pFGET19_Ulp1 (#64697) is a new version of pHYRS52(#31122) with kanamycin resistance.
origin :
37
pi :
alt_names
ubiquitin-like protein-specific protease 1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL020C, NIB1
geneULP1
id856087
genbank_ids
mutationUlp1(403-621)
nameUlp1
shRNA_sequence
size660
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagHis
resistance markers :
221
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA