CMV-218_HNF4A2 | Addgene 31090 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CMV-218_HNF4A2

catalog :

31090

citations: 1

Reference
Senkel S, Waldner C, Ryffel G, Thomas H. Improved conditional expression systems resulting in physiological level of HNF4alpha expression confirm HNF4alpha induced apoptosis in the pancreatic beta-cell line INS-1. BMC Res Notes. 2009;2:210 pubmed publisher

product information

Catalog Number :

31090

Product Name :

CMV-218_HNF4A2

article :

doi	10.1186/1756-0500-2-210
id	4331
pubmed_id	19835622

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA5/FRT/TO

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5150

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Other

Flp/FRT

growth notes :

for Flp mediated site directed integration, tetracycline inducibilty by Tet operator in CMV promoter shortened to -218. Corresponding wild type (full length) CMV promoter construct in FR_HNF4A2 (Thomas et al 2004).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF-14, TCF14
gene	HNF4A
id	3172

genbank_ids

mutation

splice variant 2 of HNF4A, deletion of CMV promoter to -218.

name

HNF4A

shRNA_sequence

size

1400

species

9606

Homo sapiens