pET-3c-rhVTN-N | Addgene 30225 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET-3c-rhVTN-N

catalog :

30225

citations: 1

Reference
Chen G, Gulbranson D, Hou Z, Bolin J, Ruotti V, Probasco M, et al. Chemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011;8:424-9 pubmed publisher

product information

Catalog Number :

30225

Product Name :

pET-3c-rhVTN-N

article :

doi	10.1038/nmeth.1593
id	4239
pubmed_id	21478862

bacterial resistance :

Ampicillin

cloning :

backbone

pET-3c

backbone_mutation

backbone_origin

Novagen

backbone_size

4607

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Note that the first 19 amino acids are the signal peptide for VTN, which is cleaved off on the mature protein during normally protein synthesis in mammalian cells. That means wild type mature VTN starts from aa20. That is why none of the four constructs have amino acid 1-19 and why pET-3c-rhVTN-N is a deletion of aa 20-62 from WT VTN.

growth temp :

DH5a in LB media

origin :

pi :

alt_names

VTN

rhVTN-N

VTN-N

cloning

clone_method	Restriction Enzyme
cloning_site_3	NdeI
cloning_site_5	BamHI
promoter
sequencing_primer_3	T7
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	V75, VN, VNT
gene	VTN
id	7448

genbank_ids

mutation

deleted amino acids: 20*-62

name

rhVitronectin with N terminus cleavage

shRNA_sequence

size

1254

species

9606

Homo sapiens