pCAX APP C99 | Addgene 30146 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCAX APP C99

catalog :

30146

citations: 4

Reference
Rimal S, Li Y, Vartak R, Geng J, Tantray I, Li S, et al. Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease. Acta Neuropathol Commun. 2021;9:169 pubmed publisher
Manville R, Abbott G. The Amyloid Precursor Protein C99 Fragment Modulates Voltage-Gated Potassium Channels. Cell Physiol Biochem. 2021;55:157-170 pubmed publisher
Swaminathan G, Zhu W, Plowey E. BECN1/Beclin 1 sorts cell-surface APP/amyloid ? precursor protein for lysosomal degradation. Autophagy. 2016;12:2404-2419 pubmed
Young Pearse T, Bai J, Chang R, Zheng J, LoTurco J, Selkoe D. A critical function for beta-amyloid precursor protein in neuronal migration revealed by in utero RNA interference. J Neurosci. 2007;27:14459-69 pubmed

product information

Catalog Number :

30146

Product Name :

pCAX APP C99

article :

doi	10.1523/JNEUROSCI.4701-07.2007
id	4221
pubmed_id	18160654

bacterial resistance :

Ampicillin

cloning :

backbone

pCAX

backbone_mutation

backbone_origin

backbone_size

4731

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The insert contains an additional 83 amino acids of unknown composition on the n-terminus. It is unknown whether these amino acids affect plasmid function.

origin :

pi :

alt_names

Amyloid Precursor Protien fragment

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	XhoI
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4
gene	APP
id	351

genbank_ids

mutation

C-terminal from BACE site of APP

name

APP C99

shRNA_sequence

size

297

species

9606

Homo sapiens