This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAd/WT1-IRES-nAmCyan
catalog :
29756
citations: 3
Reference
Chebly A, Ropio J, Peloponese J, Poglio S, Prochazkova Carlotti M, Cherrier F, et al. Exploring hTERT promoter methylation in cutaneous T-cell lymphomas. Mol Oncol. 2021;: pubmed publisher
Tan H, Wang N, Li S, Hong M, Guo W, Man K, et al. Repression of WT1-Mediated LEF1 Transcription by Mangiferin Governs β-Catenin-Independent Wnt Signalling Inactivation in Hepatocellular Carcinoma. Cell Physiol Biochem. 2018;47:1819-1834 pubmed publisher
Wang N, Tan H, Chan Y, Guo W, Li S, Feng Y. Identification of WT1 as determinant of heptatocellular carcinoma and its inhibition by Chinese herbal medicine Salvia chinensis Benth and its active ingredient protocatechualdehyde. Oncotarget. 2017;8:105848-105859 pubmed publisher
product information
Catalog Number :
29756
Product Name :
pAd/WT1-IRES-nAmCyan
article :
doi
id4196
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepAd/CMV/V5-DEST
backbone_mutation
backbone_originInvitrogen
backbone_size36686
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that Addgene distributes this plasmid in DH5a, but the depositing lab maintained the plasmid in OmniMAX 2 T1R (Invitrogen catalogue #C8540-03)
origin :
37
pi :
alt_names
WT1
cloning
clone_methodGateway Cloning
cloning_site_3attB2
cloning_site_5attB1
promoter
sequencing_primer_3V5
sequencing_primer_5attB1
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAWT1, GUD, NPHS4, WAGR, WIT-2, WT-1, WT33
geneWT1
id7490
genbank_ids
BC032861
mutationNo mutations; AmCyan is nuclear localized; WT1 and nAmCyan are separated by IRES; AmCyan from Clontech
nameWilms tumor 1
shRNA_sequence
size2300
species
9606
Homo sapiens
tags
location
tagIRES
location
tagNuclear localization sequence
locationC terminal on insert
tagAmCyan
plasmid copy :
WT1 - Thermo Scientific/OpenBiosystems AmCyan - Clontech
resistance markers :
951
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA