pET23b-proMMP-7-E215Q | Addgene 29642 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET23b-proMMP-7-E215Q

catalog :

29642

citations: 1

Reference
Ra H, Harju Baker S, Zhang F, Linhardt R, Wilson C, Parks W. Control of promatrilysin (MMP7) activation and substrate-specific activity by sulfated glycosaminoglycans. J Biol Chem. 2009;284:27924-32 pubmed publisher

product information

Catalog Number :

29642

Product Name :

pET23b-proMMP-7-E215Q

article :

doi	10.1074/jbc.M109.035147
id	4153
pubmed_id	19654318

bacterial resistance :

Ampicillin

cloning :

backbone

pET23b

backbone_mutation

backbone_origin

EMD Biosciences

backbone_size

3700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

MMP7 sequence in this plasmid is suitable for expression in bacteria but NOT in mammalian cells, as the signal sequence is not included. The MMP7 amino acid sequence starts at amino acid 18 when compared to GenBank accession number NP_002414.1. Note that a superfluous methionine corresponding to the start signal for translation is present at the N-terminus of the expressed protein Please note that the His tag present in the vector is not in-frame with MMP-7

growth temp :

Plasmid provided in BL21 RIL cells Not Codon Plus

origin :

pi :

alt_names

Promatrilysin

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	T7-term
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MMP-7, MPSL1, PUMP-1
gene	MMP7
id	4316

genbank_ids

mutation

E215Q Catalytically Inactive

name

proMMP-7

shRNA_sequence

size

species

9606

Homo sapiens