This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MKK2
catalog :
29580
citations: 2
Reference
Banks A, McAllister F, Camporez J, Zushin P, Jurczak M, Laznik Bogoslavski D, et al. An ERK/Cdk5 axis controls the diabetogenic actions of PPARγ. Nature. 2015;517:391-5 pubmed publisher
Hari S, Merritt E, Maly D. Sequence determinants of a specific inactive protein kinase conformation. Chem Biol. 2013;20:806-15 pubmed publisher
product information
Catalog Number :
29580
Product Name :
MKK2
article :
doi10.1016/j.chembiol.2013.05.005
id8560
pubmed_id23790491
bacterial resistance :
Ampicillin
cloning :
backbonepMCSG10
backbone_mutation
backbone_origin
backbone_size5961
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Addgene plasmid# 21194 was used as template for MKK2. Ligation-independent-cloning.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3T7-term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFC4, MAPKK2, MEK2, MKK2, PRKMK2
geneMAP2K2
id5605
genbank_ids
mutationConstitutively active mutant; Starting Met removed; Deletion of EQQKKRLE, residues 48 to 55; S222D and S226D mutations present
nameMKK2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHIS6
locationN terminal on backbone
tagGST
locationN terminal on backbone
tagTEV
resistance markers :
943
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA