Lenti-sh1368 knockdown c-myc | Addgene 29435 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Lenti-sh1368 knockdown c-myc

catalog :

29435

citations: 11

Reference
Chun S, Fortin B, Fellows R, Habowski A, Verlande A, Song W, et al. Disruption of the circadian clock drives Apc loss of heterozygosity to accelerate colorectal cancer. Sci Adv. 2022;8:eabo2389 pubmed publisher
Luanpitpong S, Poohadsuan J, Klaihmon P, Kang X, Tangkiettrakul K, Issaragrisil S. Metabolic sensor O-GlcNAcylation regulates megakaryopoiesis and thrombopoiesis through c-Myc stabilization and integrin perturbation. Stem Cells. 2021;: pubmed publisher
Krishna G, Soman Pillai V, Valiya Veettil M. Upregulation of GLS1 Isoforms KGA and GAC Facilitates Mitochondrial Metabolism and Cell Proliferation in Epstein-Barr Virus Infected Cells. Viruses. 2020;12: pubmed publisher
Luanpitpong S, Rodboon N, Samart P, Vinayanuwattikun C, Klamkhlai S, Chanvorachote P, et al. A novel TRPM7/O-GlcNAc axis mediates tumour cell motility and metastasis by stabilising c-Myc and caveolin-1 in lung carcinoma. Br J Cancer. 2020;: pubmed publisher
Best S, Hashiguchi T, Kittai A, Bruss N, Paiva C, Okada C, et al. Targeting ubiquitin-activating enzyme induces ER stress-mediated apoptosis in B-cell lymphoma cells. Blood Adv. 2019;3:51-62 pubmed publisher
Liang K, Smith E, Aoi Y, Stoltz K, Katagi H, Woodfin A, et al. Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy. Cell. 2018;175:766-779.e17 pubmed publisher
Luanpitpong S, Angsutararux P, Samart P, Chanthra N, Chanvorachote P, Issaragrisil S. Hyper-O-GlcNAcylation induces cisplatin resistance via regulation of p53 and c-Myc in human lung carcinoma. Sci Rep. 2017;7:10607 pubmed publisher
Rodina A, Wang T, Yan P, Gomes E, Dunphy M, Pillarsetty N, et al. The epichaperome is an integrated chaperome network that facilitates tumour survival. Nature. 2016;538:397-401 pubmed publisher
Patel P, Suram A, Mirani N, Bischof O, Herbig U. Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence. Proc Natl Acad Sci U S A. 2016;113:E5024-33 pubmed publisher
Valiya Veettil M, Dutta D, Bottero V, Bandyopadhyay C, Gjyshi O, Sharma Walia N, et al. Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposi's sarcoma-associated herpesvirus infection promotes cell proliferation. PLoS Pathog. 2014;10:e1004389 pubmed publisher
Lin C, Jackson A, Guo J, Linsley P, Eisenman R. Myc-regulated microRNAs attenuate embryonic stem cell differentiation. EMBO J. 2009;28:3157-70 pubmed publisher

product information

Catalog Number :

29435

Product Name :

Lenti-sh1368 knockdown c-myc

article :

doi	10.1038/emboj.2009.254
id	4123
pubmed_id	19745813

bacterial resistance :

Ampicillin

cloning :

backbone

FUGW, modified

backbone_mutation

backbone_origin

David Baltimore (Caltech) and Valerie

backbone_size

7000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

growth notes :

sh1368 sequence= GACGAGAACAGTTGAAACA The FUGW modified vector was obtained from Dr. Valera Vasioukhin at FHCRC (Lien et al., 2008). To generate the lentiviral vector for production of shRNA, the FUGW vector (provided by D. Baltimore, California Institute of Technology, Pasadena, CA; Lois et al., 2002) was modified by placing internal ribosome entry site neomycin sequences downstream from EGFP and cloning of human H1 promoter followed by unique BamHI EcoRI sites upstream from the ubiquitin promoter. Please note that the insert was cloned into BamHI-EcoRI sites using BglII-EcoRI fragment. The BamHI site was destroyed after ligation. See the attached document for a map from Lien et al., 2008 for vector information.

growth temp :

Stbl3

inserts :

Unknown

origin :

pi :

alt_names

c-Myc

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	CMV-Fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

genbank_ids

mutation

name

c-Myc shRNA

shRNA_sequence

size

species

9606

Homo sapiens