This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEMS1595
catalog :
29272
citations: 2
Reference
Teng X, Liu Y, Wang L, Wang G. Lidocaine exerts anticancer activity in bladder cancer by targeting isoprenylcysteine carboxylmethyltransferase (ICMT). Transl Androl Urol. 2021;10:4219-4230 pubmed publisher
Portales Casamar E, Swanson D, Liu L, de Leeuw C, Banks K, Ho Sui S, et al. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010;107:16589-94 pubmed publisher
product information
Catalog Number :
29272
Product Name :
pEMS1595
article :
doi10.1073/pnas.1009158107
id4093
pubmed_id20807748
bacterial resistance :
Ampicillin
cloning :
backbonepEMS1313
backbone_mutation
backbone_origin
backbone_size18843
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Pleiades Promoter Project [sic, Pleaides Plieades]
growth notes :
Insert was not sequence confirmed, ends may be different and may contain some internal SNPs (sequence is based on initial cloning design, and not necessarily final cloning design) Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory by a 14 nucleotide insertion at bp#3742/3743. This insertion is not known to affect the function of the plasmid.
growth strain :
Expression of the reporter gene was not detected in the brain and eye using LacZ.
growth temp :
DH10B
origin :
37
pi :
alt_names
ICMT
isoprenylcysteine carboxyl methyltransferase
cloning
clone_methodRestriction Enzyme
cloning_site_3 MluI
cloning_site_5AvrII
promoter
sequencing_primer_3
sequencing_primer_5Unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSTE14, MST098, MSTP098, PCCMT, PCMT, PPMT
geneICMT
id23463
genbank_ids
mutation
namePle124
shRNA_sequence
size3569
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagIntron-LacZ-NLS
resistance markers :
928
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA