This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEMS1424
catalog :
29220
citations: 3
Reference
Korecki A, Cueva Vargas J, Fornes O, Agostinone J, Farkas R, Hickmott J, et al. Human MiniPromoters for ocular-rAAV expression in ON bipolar, cone, corneal, endothelial, Müller glial, and PAX6 cells. Gene Ther. 2021;28:351-372 pubmed publisher
de Leeuw C, Dyka F, Boye S, Laprise S, Zhou M, Chou A, et al. Targeted CNS Delivery Using Human MiniPromoters and Demonstrated Compatibility with Adeno-Associated Viral Vectors. Mol Ther Methods Clin Dev. 2014;1:5 pubmed
Portales Casamar E, Swanson D, Liu L, de Leeuw C, Banks K, Ho Sui S, et al. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010;107:16589-94 pubmed publisher
product information
Catalog Number :
29220
Product Name :
pEMS1424
article :
doi10.1038/s41434-021-00227-z
id28211774
pubmed_id33531684
bacterial resistance :
Ampicillin
cloning :
backbonepEMS1306
backbone_mutation
backbone_origin
backbone_size16454
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
High copy number homologous recombination plasmid for gene targeting at the mouse Hprt locus.
growth notes :
Additional References: 1 Korecki, A. J. et al. Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye and Brain, Including Seventeen Carrying a New Inducible-First Constitutive-Ready Allele. Genetics 211, 1155-1177 (2019). 2 de Leeuw, C. N. et al. rAAV-Compatible MiniPromoters for Restricted Expression in the Brain and Eye. Molecular Brain 9, 52 (2016). 3 Scalabrino, M. L. et al. Intravitreal delivery of a novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital stationary night blindness. Hum Mol Genet 24, 6229-6239, doi:10.1093/hmg/ddv341, ddv341 [pii] (2015). 4 de Leeuw, C. N. et al. Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Mol Ther Methods Clin Dev 1, 1-15 (2014).
growth strain :
The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
growth temp :
DH10B
origin :
37
pi :
alt_names
PCP2
Purkinje cell protein 2
cloning
clone_methodRestriction Enzyme
cloning_site_3MluI
cloning_site_5SacI
promoterMiniPromoter Ple155 from the human PCP2 gene
sequencing_primer_3
sequencing_primer_5Unknown
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namePle155-EGFP-NLS
shRNA_sequence
size1652
species
tags
resistance markers :
928
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA