pEMS1253 | Addgene 29129 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEMS1253

catalog :

29129

citations: 1

Reference
Portales Casamar E, Swanson D, Liu L, de Leeuw C, Banks K, Ho Sui S, et al. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010;107:16589-94 pubmed publisher

product information

Catalog Number :

29129

Product Name :

pEMS1253

article :

doi	10.1073/pnas.1009158107
id	4093
pubmed_id	20807748

bacterial resistance :

Ampicillin

cloning :

backbone

pEMS1307

backbone_mutation

backbone_origin

backbone_size

16482

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Pleiades Promoter Project [sic, Pleaides Plieades]

growth notes :

Insert was not sequence confirmed, ends may be different and may contain some internal SNPs (sequence is based on initial cloning design, and not necessarily final cloning design) Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory by a 2 nucleotide deletion at bp#4123 & 4124. This deletion is not known to affect the function of the plasmid.

growth strain :

Expression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.

growth temp :

DH10B

origin :

pi :

alt_names

HBEGF

heparin-binding EGF-like growth factor

cloning

clone_method	Restriction Enzyme
cloning_site_3	AscI
cloning_site_5	FseI
promoter
sequencing_primer_3
sequencing_primer_5	Unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DTR, DTS, DTSF, HEGFL
gene	HBEGF
id	1839

genbank_ids

mutation

name

Ple108

shRNA_sequence

size

3962

species

9606

Homo sapiens